| abstract |
The invention discloses a preparation method of a duck natural immunopotentiator based on a traditional Chinese medicine extract, which comprises the following steps: (1) establishing a rutin standard curve; (2) extracting astragalus flavone; (3) and (4) calculating the concentration of the astragalus flavone. The duck natural immunopotentiator based on the traditional Chinese medicine extract provided by the invention can obviously increase the levels of duck serum cytokines IL-1 beta, IFN-alpha and IFN-beta, up-regulate the tissue expression levels of natural immunity related genes RIG-I, TLR3, TLR7, IFN-alpha, IFN-beta, IL-1 beta and TNF-alpha, inflammation related genes COX2 and iNOS, and apoptosis related gene Caspase3 in duck immune organs liver, spleen, thymus and bursa of fabricius, and enhance the natural immune response and disease resistance of ducks. |