| abstract |
The invention provides a method and a kit for quickly preparing a DNA sequencing library by using efficient ligation technology, wherein the method includes the following steps: DNA 3' adapter connection, complementary chain synthesis, DNA 5' adapter connection and library amplification. The kit contains the following components: tool enzyme, tool enzyme reaction system, linker system, extension system, PCR reaction system and Low‑EDTA‑TE. The invention realizes the successful building of various trace DNA samples, breaks the conventional means of building a library, avoids the loss of DNA caused by end repair, and reflects the true information of the samples as much as possible. |