http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110607267-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate | 2019-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110607267-B |
| titleOfInvention | Sheep listeria balanced lethal system, construction method and application |
| abstract | The invention relates to a sheep Listeria balanced lethal system, a construction method and application, wherein an attenuated nutritional deletion strain (LI delta actAplcB delta dat) with four deleted genes (actA, plcB, dal and dat) is obtained by utilizing a homologous recombination method on the basis of an actA and plcB double-gene attenuated LI strain, the attenuated nutritional deletion strain cannot grow in a nutritional environment without adding D-alanine, and a dal gene complementation plasmid is constructed and introduced into the strain, so that an LI balanced lethal system, namely a complementation strain (LI delta actAplcB delta dat: dal) is obtained. The LI balanced lethal system obtained by the invention is stable genetically. The complementation plasmid contained in the bacterial cell of the LI balance lethal system obtained by the invention can be used as a vector for inserting exogenous antigen genes, so that the system can stably carry and express the exogenous genes, and the LI can be used as a vector for preparing vaccines by utilizing the biological characteristics related to antigen presentation of the LI. |
| priorityDate | 2019-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 87.