| abstract |
The invention discloses a recombinant human type III collagen α1 chain and its application. The nucleotide sequence encoding the recombinant human type III collagen α1 chain is shown in SEQ ID NO: 1. The recombinant human The source type III collagen α1 chain contains, from the amino terminal, an amino terminal affinity purification tag, a human source type III collagen mature peptide chain, and a carboxy terminal affinity purification tag. The recombinant human type III collagen according to the embodiment of the present invention is designed with specific affinity purification tags at both ends of the mature peptide chain of human type III collagen, which is beneficial to the purification of the full-length protein and can also be used for collagen identification, and the recombinant human type III collagen according to the embodiment of the present invention uses the mature peptide chain of type III collagen, removes the propeptide at both ends, and the expression level is much higher than that of the gene containing the propeptide. |