| abstract |
The invention discloses a herbicide hapten, an artificial antigen and an antibody as well as a preparation method and application thereof. The herbicide hapten provided by the invention not only retains the characteristic structure of the herbicide to the greatest extent, but also significantly enhances the immunogenicity of the herbicide hapten. , and has an aldehyde group that can be coupled with the carrier protein; using the herbicidal ether artificial antigen obtained by coupling the herbicidal ether hapten with the carrier protein to immunize animals is more conducive to stimulating the animal immune response to produce stronger specificity and higher sensitivity With high antibody, the sensitivity of the detected herbicide antibody can reach 0.1 μg/L, and the cross-reaction rate with other pesticides is low, which provides a basis for the subsequent establishment of various immunoassay methods for herbicide. |