| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_18d92c46f60af8af24e262b67381d7eb |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-185
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-154 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate |
2018-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e421a40bb6560bfd11074ed78df15353
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b69ed56ba8b9619ababda47fde838212
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff4a1917571aaea0053b2955f6229dc3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_746b214ec009864d782334873dd17142
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a706926c5c3cb30b022a6c04d4365bda
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d067450c5af00f8866ee015a7e570f47 |
| publicationDate |
2019-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-110582580-A |
| titleOfInvention |
Strand-specific detection of bisulfite-converted duplexes |
| abstract |
BiSeqS (bisulfite sequencing system) is a technology that can improve sequencing specificity by at least two orders of magnitude over that achieved by molecular barcodes and can be applied to any massively parallel sequencing instrument. BiSeqS uses bisulfite treatment to distinguish between the two strands of molecularly barcoded DNA. Its specificity stems from the requirement for the same mutation to be identified in both chains. Because no library preparation is required, this technique allows very efficient use of template DNA as well as sequence reads, which are almost entirely restricted to the amplicon of interest. This high efficiency is critical for clinical samples such as plasma, from which only trace amounts of DNA are usually obtained. BiSeqS can be used to assess transversions, as well as small insertions or deletions, and can reliably detect one mutation in >10,000 wild-type molecules. |
| priorityDate |
2017-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |