http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110577984-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_79b0f0cba091ed466f711f503b7114fd |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate | 2019-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3fca2509c2e72c1d3f1aca5e208b7104 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_273fd6b8e3453a9f06e40b9984b31dca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ecfcf798384f1e9c93708a3ed7a4d32e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f31c436b97fd288751b836bd9c74d604 |
| publicationDate | 2019-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110577984-A |
| titleOfInvention | A multiple visualization nucleic acid detection method |
| abstract | The invention belongs to the field of gene detection, and discloses a multiple visualization nucleic acid detection method, comprising the following steps: 1) respectively designing corresponding padlock probe sequences and universal primer sequences for different target nucleic acid molecules, the padlock probe sequences Contains gene-specific sequences and universal primer recognition sequences; 2) Add padlock probes to the solution to be detected, add ligase and reaction buffer at the same time, and perform linkage reactions in the same reaction system; 3) Distribute the linkage reaction products In different reaction tubes, the reaction tubes respectively contain universal primers paired with the universal primer recognition sequence on the padlock probe, and perform hyperbranched rolling circle amplification reaction in the reaction tube to obtain the amplification product; 4) Visualization Reaction: Add micro-nano particles, and observe the test results according to whether agglomeration occurs. The method of the invention has low detection cost, simple operation steps and high sensitivity, and can realize high-throughput detection of various nucleic acids. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113189323-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113189323-B |
| priorityDate | 2019-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.