| abstract |
The invention discloses a method for producing glucosamine by Escherichia coli fermentation. After activating the seeds of Escherichia coli, Bacillus subtilis and Staphylococcus a mixed starter is prepared, and the mixed starter is inoculated into a fermentation medium containing glucose and a nitrogen source Medium constant temperature shaker culture, the fermentation process is carried out by constant feeding method, the pH of the fermentation is controlled by ammonia water to 6.8-7.0, and the fermentation liquid containing glucosamine is obtained, and the glucosamine is obtained after separation and purification. The invention improves the fermentation activity of Escherichia coli by mixing the starter, and the addition of cysteine and alanine can reduce the production speed of glutamic acid in the fermentation process and increase the synthesis rate of glucosamine. |