| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aaaaf4049c38c48aea4f1320fa64fb20 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15043
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-32151 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70596
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0645
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate |
2019-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70dd8e8dd2c4c92d006575cdf9cb8422
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_021e3f124abdccf66df8e5923ccc7dbd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1230ac9992ff47c42b38aa4347eaa83a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2bfef549cb5867a693dc45ac0e9ca657
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_29cefe3dcf371e42c1c5b1f86a7095b9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf96b762f6f5310ad385a785c9a43e4c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab81faa58124f3f4b33d977ebf98605b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1924e6037d18a1710f8ffda855a9b32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21a9355e2092c61e69a2ac9835423375
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f606aac58acbcd2ac26a6da36ab0611 |
| publicationDate |
2019-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-110577934-A |
| titleOfInvention |
Construction method and application of TLR4 gene knocked-down porcine alveolar macrophage cell line |
| abstract |
The invention relates to the technical field of biology, in particular to a construction method of a TLR4 gene knocked-down porcine alveolar macrophage cell line and application thereof. The preservation number of the porcine alveolar macrophage cell line is as follows: CCTCC NO: C2019174. the construction method of the TLR4 gene knocked-down porcine alveolar macrophage cell line comprises the following steps: transfecting porcine alveolar macrophages with recombinant lentiviruses containing sgRNA, and obtaining the TLR4 gene-knocked-down porcine alveolar macrophage system through resistance screening; the sgRNA is obtained by annealing a nucleotide sequence shown in SEQ ID NO. 1-2. The TLR4 knocked-down gene porcine alveolar macrophage cell line constructed by the invention can promote replication of foot-and-mouth disease viruses, and compared with an undeknocked cell line, the replication amount of the foot-and-mouth disease viruses in the TLR4 knocked-down gene porcine alveolar macrophage cell line is 2-3 times that of the same. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115948466-A |
| priorityDate |
2019-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |