| abstract |
The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, and polynucleotides encoding engineered phenylalanine ammonia lyase (PAL) polypeptides. In some embodiments, engineered PAL polypeptides are optimized to provide enhanced catalytic activity, as well as reduced susceptibility to proteolysis and increased tolerance to storage at elevated temperatures. In some embodiments, an engineered PAL polypeptide comprises fewer phenylalanine residues than a wild-type PAL polypeptide. The present invention also provides methods for using compositions comprising engineered PAL polypeptides for therapeutic and industrial purposes. |