http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110564755-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y601-01013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 |
| filingDate | 2019-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110564755-B |
| titleOfInvention | Method for preparing (S) -3-phenyl-1, 2-epoxypropane and derivatives thereof by using biological enzyme catalysis |
| abstract | The invention provides a method for preparing (S) -3-phenyl-1, 2-epoxypropane and derivatives thereof by using biological enzyme catalysis, which belongs to the technical field of compound synthesis and comprises the following steps: 1) synthesizing an HhAPL gene, and inserting the HhAPL gene into a multiple cloning site of a pET24(a) vector to obtain an expression vector pET-Apl; 2) transferring the expression vector pET-Apl into escherichia coli to obtain a recombinant cell for expressing the HhAPL gene; 3) preparing the recombinant cells into a bacterial suspension of 50-200 g/L by using a buffer solution; 4) and mixing the bacterial suspension, the substrate auxiliary solvent and the substrate to obtain a reaction system, and reacting to obtain a target product. The bio-enzyme used in the method of the invention catalyzes the asymmetric epoxidation of olefin to have higher enantioselectivity and catalytic reaction activity. |
| priorityDate | 2019-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 565.