http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110564634-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-485 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2018-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110564634-B |
| titleOfInvention | Engineering bacterium for extracellularly secreting and expressing inonotus obliquus dipeptidase |
| abstract | An engineering bacterium for extracellularly secreting and expressing the dipeptidase of inonotus obliquus is obtained by operably connecting a cloned dipeptidase gene IDP of an transmembrane region of the inonotus obliquus with an expression vector to obtain a recombinant expression vector capable of expressing the protein and introducing the recombinant expression vector into a proper host cell. The invention also provides a method for preparing the IDP, which is to culture IDP-X33 and obtain the dipeptidase IDP of the fuscoporia obliqua transmembrane area through methanol induction expression. The culture conditions were 28 ℃ and initial pH 7, and the culture was carried out at 200rpm for 96 hours. The induction conditions were supplemented with the inducer methanol every 24 hours to a final methanol concentration of 1.0%. The recombinant pichia pastoris expression dipeptidase method provided by the invention has the extracellular secretory enzyme activity of 24U/mL and the cell density (OD) 600 ) The secretion efficiency of the enzyme was 24.5, and 1U/mL. |
| priorityDate | 2018-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 310.