| abstract |
The invention provides a method for preparing an animal model of liver cancer, which includes the following specific steps: (1) select 4-week-old ICR mice, observe and raise them for 2 weeks; place HepG2 cells in DMEM medium supplemented with 10% fetal bovine serum After culture, passage and expansion, inject into the peritoneal cavity of ICR mice with a syringe; (2) After the mice have obvious ascites, draw out 2 mL of ascites, add it to a centrifuge tube, centrifuge, and discard the supernatant; add 5 mL of PBS solution to resuspend, rinse, and then centrifuge Discard the supernatant for 5 minutes; (3) Repeat the washing with PBS solution for 2‑3 times in the same way as in step (2); (4) Add 5 mL of PBS solution to the precipitate after discarding the supernatant in step (3) to blow Scatter the cells and centrifuge for 3 minutes; use a precision pipette to take out the supernatant and the red blood cells in the upper layer of the precipitate, and keep the white precipitate in the lower layer; the effect of the primary liver cancer mouse model prepared by the present invention is much better than that of the existing primary liver cancer The animal model establishment method is more authentic, practical, convenient and economical than existing models. |