http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110551803-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7aac4b596c9b7116d73db78d579683e4 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2019-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c49a16fbbdd2736b0789cae3f8fea6de http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d1ef54c32db9785589be2c92334beef |
| publicationDate | 2019-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110551803-A |
| titleOfInvention | A sequencing method for human HLA gene polymorphism primer insertion or deletion |
| abstract | The invention discloses a sequencing method for insertion or deletion of human HLA gene polymorphism primers, which belongs to the technical field of HLA gene sequencing. A sequencing method for insertion or deletion of human HLA gene polymorphism primers comprises the following steps: extracting DNA, Collect fasting peripheral venous blood samples from the target subjects, use DNA extraction kits to extract genomic DNA from the blood samples, measure DNA concentration and purity with an ultraviolet spectrophotometer, and denature at high temperature to dissociate the double strands of the DNA template into DNA single strands. The invention combines PCR amplification technology with fluorescent markers, takes improving the purity and concentration of DNA as the primary premise in the sequencing process, and utilizes the advantages of PCR amplification technology and fluorescent markers to minimize the difficulties and problems in the sequencing process. Defects, the sequencing process has high sensitivity, strong specificity, and easy operation, thereby ensuring the sensitivity and accuracy of HLA gene sequencing, and accurately obtaining whether there are insertions or deletions in the HLA gene. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022116456-A1 |
| priorityDate | 2019-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.