http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110551769-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y203-03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1025 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-22 |
| filingDate | 2019-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110551769-B |
| titleOfInvention | Production method of phloroglucinol |
| abstract | The phloroglucinol has important application prospect in the fields of medical treatment, materials and the like as a precursor substance of a high-value compound. Because the traditional chemical synthesis method has defects in the aspects of cost, environmental protection and the like, the invention adopts cheap and environment-friendly acetic acid as a carbon source to realize the biosynthesis of phloroglucinol. The invention firstly constructs two synthetic routes taking acetic acid as a carbon source, verifies the feasibility of the method through fermentation, and determines that the route for expressing PhlD, AckA and Pta is a phloroglucinol production route. Respectively optimizing the acetic acid concentration and the yeast extract concentration in the M9 fermentation medium, and increasing the phloroglucinol yield to 228 mg/L. The feasibility of using CRISPR/dCas9 for competitive pathway inhibition was demonstrated using the green fluorescent protein eGFP. And then fermentation is carried out, the expression level of the citrate synthase in the competitive pathway is reduced through CRISPR, and the phloroglucinol yield is increased to 284 mg/L. The invention provides a novel phloroglucinol production method, and provides a new strategy for the industrial production of phloroglucinol. |
| priorityDate | 2019-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 483.