| abstract |
The invention relates to a method for extracting the total RNA of Aeromonas vergei, belonging to the technical field of genetic engineering molecule extraction. Lysozyme was added at a concentration of 5 mg/ml ± 5%. Compared with the existing extraction method, the extraction method provided by the present invention significantly increases the concentration of the obtained RNA, which meets the requirements of the subsequent reverse transcription experimental system. The resulting RNA was purified by using a column-type RNA rapid concentration and purification kit, model B518688, produced by Shanghai Shenggong, indicating that gDNA (genomic DNA) contamination was eliminated, and the problem of RNA degradation was also resolved. |