http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110551707-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_615bd7f2c0a8cad77f76b71d1fb9f42a |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-50 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-50 |
filingDate | 2019-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7de1890853a0d259e5e8d77811a2e657 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4cbf7754f5dec54b4ec4543f127df8e1 |
publicationDate | 2019-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110551707-A |
titleOfInvention | A kind of method for purifying neutral or alkaline protease |
abstract | The invention discloses a method for purifying neutral or alkaline protease. The DNA sequence encoding the eglinC mutant is chemically synthesized, the recombinant expression vector of the eglinC mutant is constructed, Escherichia coli is used for recombinant expression and purification of the eglinC mutant protein, and then the eglinC mutant protein is recombined and purified. The protein is coupled to the sepharose4B column material, and the specific binding of eglinC mutant and protease is used to separate and purify bacterially expressed neutral or alkaline protease. The present invention utilizes the affinity purification system constructed by eglinC mutant, the method is simple, the operation steps are few, the loss of enzyme activity is low, and the purification multiple is high. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111500561-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114292833-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111500561-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113999832-A |
priorityDate | 2019-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 95.