http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110551668-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 |
| filingDate | 2019-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110551668-B |
| titleOfInvention | Enterotoxin gene LT-knocked-out escherichia coli and construction method thereof |
| abstract | The invention discloses a construction method of enterotoxin gene LT knocked-out escherichia coli, which comprises the following steps: 1) constructing LT gene homologous recombination donor LT donar, wherein the base sequence of LT donar sequentially comprises L-arm, cat promoter, CmR, sgCommon, PAM, L-short and R-arm polynucleotide fragments; and 2) preparing competent cells containing pCas-Red plasmids, adding the LT donar in the step 1) for transformation, and then screening positive clones to obtain the Escherichia coli with the heat-labile enterotoxin gene LT knocked out tracelessly. According to the invention, Crispr/Cas9 is combined with a lambda-Red homologous recombination system, the LT gene of enterotoxigenic Escherichia coli K88 is successfully subjected to traceless knockout, a mutant strain loses hemolytic ability, and growth is slowed down; the method can quickly and conveniently knock out enterotoxigenic Escherichia coli K88 gene without trace, and provides a solid research foundation for researching LT function. |
| priorityDate | 2019-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 78.