http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110551230-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-0003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-006 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08B37-00 |
| filingDate | 2019-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110551230-B |
| titleOfInvention | Preparation method of astragalus polysaccharide |
| abstract | The invention relates to a preparation method of astragalus polysaccharide, which comprises the following steps: 1) decocting radix astragali in water, filtering decoction, concentrating, adding ethanol into concentrated solution, and standing at room temperature; 2) filtering to remove supernatant, adding ethanol into the precipitate, filtering to remove supernatant, adding water into the precipitate, and dissolving; 3) adding ethanol into the solution to ensure that the ethanol content is 30-40%, taking supernate and adding ethanol to ensure that the ethanol content is 60-80%, standing, filtering to remove supernate, adding water into the precipitate to fix the volume, filtering, adding water into the filtrate, and ultrafiltering to obtain filtrate with the volume of 25% -30% of the fixed volume; 4) respectively passing the filtrate through a cation exchange chromatographic column and an anion exchange chromatographic column, eluting with an acetic acid-sodium acetate buffer solution, filtering the eluent through a filter membrane, performing fractional ultrafiltration through an ultrafiltration system to obtain two-stage ultrafiltrates, respectively performing ultrafiltration until the conductivity of the permeate is less than or equal to 150 mus/cm, and continuously performing ultrafiltration until the retention volume is 10-15% of the volume of the eluent; 5) freezing the two filtrates at-30 deg.C to-70 deg.C for 1-10 days, thawing at room temperature, centrifuging, concentrating the supernatant until the sugar content of the filtrate is 20% -26%, adding ethanol to the concentrated solution until the ethanol content is 85-95%, filtering to remove the supernatant, dissolving the precipitate with ethanol, filtering to remove the supernatant, drying, pulverizing, and mixing. |
| priorityDate | 2019-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.