http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110551199-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-485 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-485 |
| filingDate | 2019-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110551199-B |
| titleOfInvention | A method for improving the secretion and expression of recombinant human epidermal growth factor by engineering bacteria |
| abstract | The invention relates to a method for improving the secretion and expression of recombinant human epidermal growth factor by engineering bacteria. The method comprises the following steps: (1) inoculating the engineering bacteria frozen in glycerol into the LB liquid medium containing kanamycin, and culturing to obtain a seed culture solution; (2) inoculating the seed culture solution into a In MBL medium, culture, then add IPTG, glycine, Triton X-100, induce, obtain fermentation supernatant; and (3) isolate and purify recombinant human epidermal growth factor from described fermentation supernatant. Wherein, during induction, IPTG with a final concentration of 0.1 mM to 0.3 mM is added; the final concentration is 0.2% to 1.5% by mass of glycine, and the final concentration is 0.002% to 0.2% by mass of Triton X-100. The invention increases the secretion amount of hEGF, shortens the fermentation time, and thus reduces the cost. |
| priorityDate | 2019-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 87.