http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110541022-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2019-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110541022-B |
| titleOfInvention | Mycobacterium tuberculosis complex detection kit based on CRISPR-Cas12a system |
| abstract | The invention belongs to the technical field of nucleic acid detection, and relates to a detection kit and method for Mycobacterium tuberculosis complex based on the CRISPR-Cas12a system. The kit improves detection sensitivity by using recombinase polymerase amplification technology, and uses CRISPR-Cas12a to specifically target the Mycobacterium tuberculosis complex target sequence to activate the bypass cleavage activity of Cas12a, which can be sensitively and specifically detected from sputum Mycobacterium tuberculosis complex. The invention has the advantages of non-invasiveness, frequent and multiple detection, and fast detection speed. Compared with the current PCR detection method, the present invention does not require a thermocycler with a temperature rise and fall function, and can detect the Mycobacterium tuberculosis complex in sputum through fluorescence reading, and has the advantages of low cost, simple operation, high sensitivity and specificity. It is suitable for large-scale clinical applications. |
| priorityDate | 2019-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 302.