| abstract |
the invention discloses a preparation method for extracting total flavonoids from cactus, which is characterized by comprising the following steps: 1) grinding dry cactus leaves into coarse powder; 2) taking the coarse powder, adding 100ml of absolute ethyl alcohol, performing ultrasonic treatment for 3 times with the power of 120-200 w for 45min each time, combining extracting solutions, and recovering the ethyl alcohol to obtain a cactus extract; 3) extraction: adding a proper amount of distilled water into the cactus extract, uniformly stirring, adding the cactus extract into a chromatographic column, adjusting the flow rate to be 5ml/min, washing the cactus extract with distilled water to remove impurities, and desorbing the cactus extract by using 10-70% ethanol as a mobile phase; 4) recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone. The total flavone prepared by the method has high product purity, good quality, simple and easy process method, and is suitable for industrial production. |