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filingDate 2019-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_13b52228c361a34180d01e6c61f6dc4a
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publicationDate 2019-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110527694-A
titleOfInvention Turn the initiative and application of the heavy metal super-enriched transgenic engineering rape of S.plumbizincicola SpHMA2 and SpHMA3
abstract The present invention discloses a kind of method for creating for turning the dual-gene heavy metal super-enriched transgenic engineering rape of S.plumbizincicola SpHMA2 and SpHMA3, comprising: SpHMA2 and SpHMA3 is dual-gene in clone's S.plumbizincicola;Based on plant expression vector, conversion carrier of the building comprising strong promoter, screening-gene, SpHMA3 gene is connected to the restriction enzyme site of the conversion carrier by double digestion method, by the logical insertion conversion carrier of SpHMA2 gene, obtains dual-gene overexpression binary vector;Dual-gene overexpression binary vector is transferred in rape hypocotyls dissection by Agrobacterium tumefaciems, cultivate transgenic seedling and screens the transgenic positive plant of dual-gene polymerization expression.Breeding method provided by the invention can solve the problems such as wild heavy metal accumulation plant patience is limited, growth is slow, biomass is small, growing environment is special and heavy metal returns to.In addition, the application the present invention also provides heavy metal super-enriched transfer dual-gene plant obtained in above-mentioned method for creating in heavy metal-polluted soil reparation.
priorityDate 2019-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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