http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110527694-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ae5785ca2cfe0e4371000bd2f7c56424 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_70fbe08b7c540157200696115221f654 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B09C2101-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8259 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B09C1-105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8271 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B09C1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 |
filingDate | 2019-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_13b52228c361a34180d01e6c61f6dc4a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7bc040b22b89e22b4e17eb83f0a78e0c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e29a44218f97a6559dcc9d66873a34f0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7eafae13166e322e3ee6c63bf3d72274 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b76be38d3285d7c4b30ab05b8c9db1ea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_edd827a13bd394b57b48fe5a6802c742 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b378707e0957dd371fd92a90a20a1b0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26aef34998d26a93d0216832b8e456cf |
publicationDate | 2019-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110527694-A |
titleOfInvention | Turn the initiative and application of the heavy metal super-enriched transgenic engineering rape of S.plumbizincicola SpHMA2 and SpHMA3 |
abstract | The present invention discloses a kind of method for creating for turning the dual-gene heavy metal super-enriched transgenic engineering rape of S.plumbizincicola SpHMA2 and SpHMA3, comprising: SpHMA2 and SpHMA3 is dual-gene in clone's S.plumbizincicola;Based on plant expression vector, conversion carrier of the building comprising strong promoter, screening-gene, SpHMA3 gene is connected to the restriction enzyme site of the conversion carrier by double digestion method, by the logical insertion conversion carrier of SpHMA2 gene, obtains dual-gene overexpression binary vector;Dual-gene overexpression binary vector is transferred in rape hypocotyls dissection by Agrobacterium tumefaciems, cultivate transgenic seedling and screens the transgenic positive plant of dual-gene polymerization expression.Breeding method provided by the invention can solve the problems such as wild heavy metal accumulation plant patience is limited, growth is slow, biomass is small, growing environment is special and heavy metal returns to.In addition, the application the present invention also provides heavy metal super-enriched transfer dual-gene plant obtained in above-mentioned method for creating in heavy metal-polluted soil reparation. |
priorityDate | 2019-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.