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filingDate 2019-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110527017-B
titleOfInvention A kind of polyacrylamide gel for separation of small molecular weight proteins and preparation method thereof
abstract The invention relates to the technical field of protein polyacrylamide gel electrophoresis, in particular to a polyacrylamide gel used for separation of small molecular weight proteins, the gel comprising acrylamide, N,N-methylenebisacrylamide and ultrapure water , gel buffer, glycerol, ammonium persulfate solution and tetramethylethylenediamine, gel buffer including boric acid and Tris-HCl solution, pH of gel buffer is 7.0. The invention uses Tris-boric acid as a buffer system to prepare polyacrylamide gel, which can achieve good separation of small molecular proteins, the protein bands are clear and sharp, and the separation effect is similar to that of the commonly used small molecular protein separation gel Tris-Tricine polyacrylamide. The gel is comparable, and the separation effect is even better than that of Tris‑Tricine, which greatly reduces the cost of reagents and can achieve better separation effect.
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