| abstract |
The present invention relates to biology and medic laboratory technology fields, and in particular to a kind of experimental rat heart extracting blood method.The blood extracting method includes: that material prepares, blood process is tamed and taken to rat feeding.Method of the invention: can according to the number of requirement of experiment blood volume, blood was collected, experimental rat blood sampling volume is controllable；Approach is not easy to damage blood vessel under xiphoid-process, and blood loss is small；Fine needle Direct perforantes heart, takes blood quick；Its hetero-organization or organ are avoided contact with, the generation of haemolysis is reduced；Blood sampling volume is controlled simultaneously combines the body fluid supplement after blood sampling, can guarantee experimental mouse, blood was collected again after the recovery of certain time, the dosage for reducing experimental rat meets modern received reduction (Reduction) extensive in the world, substitution (Replacement), optimization (Refinement) and responsibility (Responsibility) 4R principles and requirements. |