| abstract |
The invention discloses a kind of rat long-chain non-coding lncRNA-lncMSTRG10078 and its resist cellular damage application, sequence as shown in SEQ ID NO.1 or have and the above sequence homology of SEQ ID NO.190%；The present invention confirms the objective reality of the sequence by PCR；Clone the sequence transcript, construct pcDNA3.0-lncMSTRG10078 over-express vector, transfect GH3 cell, the results showed that mitochondrial respiratory chain subunit, inflammation and apoptosis generation and metabolism change can significantly be regulated and controled by being overexpressed lncMSTRG10078, to resist cellular damage effect；Can be seen that by streaming result be overexpressed lncMSTRG10078 can the significant generation of inhibitory activity oxygen (ROS) and the generation of Apoptosis, further confirm that the effect of cell resistance cellular damage can be enhanced in the sequence. |