http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110511874-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y103-99026 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1085 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P23-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y502-01012 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y205-01029 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-89 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P23-00 |
| filingDate | 2019-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110511874-B |
| titleOfInvention | Alternarythromycin high-yield engineering bacterium based on Dunaliella metabolic pathway and construction method and application thereof |
| abstract | The invention discloses an engineering bacterium for high yield of neurosporene based on a metabolic pathway of dunaliella and a construction method and application thereof, relating to the field of genetic engineering bacteria. The invention realizes the construction of engineering bacteria by using genes of carotenoid pathway from Dunaliella (such as Dunaliella bardawil or Dunaliella salina) for the first time. When the Dunaliella is the Dunaliella bardawil, the yield of the Alternarypsin high-yield engineering bacteria is 2.4mg/g (cell dry weight); when the Dunaliella is Dunaliella salina, the yield of the Alternarypsin high-yielding engineering bacteria is 3.1mg/g (dry cell weight). The promoters of the vectors adopted by the invention are all T7 promoters, contain lacZ regulatory elements, and can be highly expressed in IPTG-acting escherichia coli BL21(DE3), which is beneficial to the mass accumulation of neurosporene and can be used for practical application and production. |
| priorityDate | 2019-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 765.