http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110501293-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_819d2d00412d671253120d16a2f09908 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-31 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 |
| filingDate | 2018-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64484fb87c4c6df0d3d3c2c3c2792afb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d858e41c231a7b0cbbe7777b101800bb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b0f4dcb14c2e34bf02fe6ec1dae874b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4743e4a734df7bbd1e903c35dd772733 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21c4386b77ebbea7d4ce12e2bcb5f076 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5428981918801c3c18c90d380d06fec2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_34b070fcc2fe381016671232334c0125 |
| publicationDate | 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110501293-A |
| titleOfInvention | Ethanol detection method and kit |
| abstract | The invention discloses an ethanol detection method and a kit. The method utilizes that when ethanol is oxidized by alcohol dehydrogenase to generate acetaldehyde, oxidized coenzyme I generates reduced coenzyme I, and the generated reduced coenzyme I converts potassium ferricyanide into potassium ferrocyanide, potassium ferrocyanide Prussian blue is produced with ferric chloride, and the amount of Prussian blue produced is determined by measuring the absorbance of Prussian blue, and the ethanol content is calculated. The kit of the invention consists of a mixed solution of 0.2-2U alcohol dehydrogenase, 0.2-2U NADH dehydrogenase, 0.2-2mM oxidized coenzyme I and 10-100mM potassium ferricyanide, buffer and 10-100mM trichloride iron. The invention can measure the ethanol concentration in the liquid in a short time only by using a microplate reader that only supports the wavelength of visible light, and the detection limit is as low as 0.1 mM. |
| priorityDate | 2018-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 288.