http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110499361-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_97a714fcb755e3262636236d7856f6b3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2019-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_762ed00307f0f55aa561a819abc13399 |
| publicationDate | 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110499361-A |
| titleOfInvention | Preparation method and application of a terminal base flow cytometric fluorescent sequencing microsphere |
| abstract | The invention discloses a preparation method and application of terminal base flow fluorescent sequencing microspheres. The method utilizes the complementary sequence of nucleic acid fragments to be tested, primers, microspheres, nucleic acid polymerase, fluorescent marker ddNTP, and ribose 3'-OH substrate. Protected fluorescently labeled dNTPs, ribose 3'‑OH protected dNTPs, ribose 3'‑OH deprotectors, etc., through the single-base extension of microsphere-coated primers, a series of nucleic acids suitable for flow cytometry detection have been obtained. The terminal bases of the base sequence are flow-type fluorescent sequencing microspheres, and these sequencing microspheres are further detected by a flow cytometer to obtain the base sequence of the nucleic acid fragment to be tested. Compared with the existing sequencing method, the present invention has obvious advantages such as brevity, accuracy and easy interpretation of data. The sequencing microspheres obtained in the present invention can be widely used in the fields of nucleic acid sequencing such as gene detection, microbial inspection, genetics, exons, single nucleotide polymorphism (SNP), genomics and proteomics, especially in tumor-associated There are good applications in the field of diagnosis. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021018080-A1 |
| priorityDate | 2019-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 95.