http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110499350-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/D01C3-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43586 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/D01C3-02 |
| filingDate | 2019-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110499350-B |
| titleOfInvention | Sericin hydrolysis method, product and application thereof |
| abstract | The invention provides a sericin hydrolysis method, belonging to the technical field of biology. The method provided by the invention comprises the following steps: (1) dissolving sericin in water to prepare a sericin water solution; (2) mixing the sericin aqueous solution with a buffer solution for adjusting the pH value, and adjusting the pH value of the mixed solution to 7.5-8.5 to obtain a to-be-hydrolyzed solution; (3) adding trypsin and calcium chloride into the to-be-hydrolyzed solution, and carrying out primary reaction for 0.5-1.5 h at the temperature of 36-38 ℃ to obtain a primary hydrolyzed solution; (4) and adding papain and sodium thiosulfate into the preliminary hydrolysate, and further reacting for 0.5-1.5 h at 48-52 ℃ to obtain the final hydrolysate. The method provided by the invention utilizes double-enzyme hydrolysis, firstly uses trypsin for treatment, and then adds papain; under the combined action of the two enzymes, the sericin can realize the hydrolysis with higher efficiency than the traditional method. |
| priorityDate | 2019-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.