http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110499316-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0631 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70585 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 2019-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110499316-B |
| titleOfInvention | Milk cow mammary gland epithelial cell line with CD44 gene knocked out and construction method thereof |
| abstract | The invention discloses a milk cow mammary gland epithelial cell line with a CD44 gene knocked out and a construction method thereof. The method utilizes a recognition sequence of gRNA for knocking out CD44 gene, and the nucleotide sequence is shown as SEQ ID NO: 1 is shown. The invention utilizes the gRNA designed by the inventor to accurately and efficiently knock out the CD44 gene, and effectively constructs the milk cow mammary epithelial cell with the CD44 gene, solves the defect of low interference efficiency in the RNAi technology, and provides a simple, convenient and efficient method for constructing a gene knock-out cell line. The CD44 gene is used as an important marker gene related to lipid metabolism and fatty acid metabolism, and the establishment of a knockout cell line provides effective gene materials and gene tools for further research on the functions of genes related to lipid metabolism and fatty acid metabolism pathways. |
| priorityDate | 2019-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 260.