http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110491447-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B25-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B30-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43595 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B30-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B25-10 |
filingDate | 2019-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110491447-B |
titleOfInvention | Codon optimization method for heterologous gene in vitro expression and application |
abstract | The invention discloses a codon optimization method for heterologous gene in vitro expression, which comprises the following steps: obtaining a nucleotide sequence of a host cell whole genome and an amino acid sequence of a whole proteome; taking the codon pairs as statistical objects, and counting the weight of each codon pair in the whole genome of the host cell; selecting a protein to be optimized, and constructing a one-way graph model with codons as nodes and a weight value between an upstream codon pair and a downstream codon pair as a line value; and obtaining the nucleotide sequence of the optimized gene according to a one-way graph model. The invention uses the whole genome and the whole proteome of a host cell as a sequence library, takes a codon pair as a statistical object, and obtains an optimal codon combination sequence by constructing a one-way graph model which takes the codon as a node and takes a weight value between an upstream codon pair and a downstream codon pair as a line value, thereby obtaining an optimized gene with an optimized nucleotide sequence, wherein the optimized gene can be efficiently expressed in vitro, and the expression quantity is obviously improved. |
priorityDate | 2019-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 281.