http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110488001-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7f564cfe89e89342df60b2878e9930d8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-531 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 |
| filingDate | 2019-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca087572a54288b34b5aa8195935f6bf |
| publicationDate | 2019-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110488001-A |
| titleOfInvention | A method of antigen retrieval is carried out using density gradient zonal centrifugation method |
| abstract | The present invention provides a kind of method for carrying out antigen retrieval using density gradient zonal centrifugation method, includes the following steps: S1. cervical cell sample collection；S2. sample rate separating liquid is added in centrifuge tube, then draws cell sample and is transferred in centrifuge tube；S3. it is centrifuged twice, centrifugation for the first time is centrifuged 2min under the effect of 200g centrifugal force, then siphons away supernatant liquid；Second of centrifugation of remaining liq is centrifuged 5min under the effect of 800g centrifugal force, finally slowly outwells supernatant；S4. the cell precipitation amount of observation centrifugation bottom of the tube, according to how much addition buffers of precipitation capacity；It is eventually fabricated cell suspension.The present invention, which innovatively uses density gradient zonal centrifugation method to carry out antigen retrieval, can also reach antigen retrieval effect same as hot repairing method under conditions of not needing high temperature and pressure.Avoiding antigen retrieval will be using the heating instrument equipment with security risk that hot repair is multiple and uses, and whole experiment process is made to compare efficient quick. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111929122-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111929122-B |
| priorityDate | 2019-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.