http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110484559-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8243 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2019-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110484559-B |
| titleOfInvention | Method for obtaining low-glucosinolate rape seeds by editing BnaC2-GMYB28 gene |
| abstract | The method for obtaining the low-glucosinolate rape seeds by editing the BnaC2-GMYB28 gene comprises the following steps: determining a key gene for synthesizing thioglycoside and determining a target gene SgRNA sequence; constructing an expression cassette; constructing a gene editing vector; transforming agrobacterium with the gene editing vector; sowing rape seeds; activating and preparing agrobacterium; preparing and dip-dyeing explants; and transferring and culturing the explants. The invention determines the endogenous gene of rape seed thioglycoside synthesis, carries out directional mutation on the endogenous gene by using a gene editing technology, obtains several single plants with different mutation types, and obviously reduces the thioglycoside of the seeds compared with a control, and the content of the thioglycoside of the T1 family seeds obtained after selfing is extremely lower than that of unedited receptor materials, thereby showing that the technology can be used for quickly obtaining the rape which has no transgenic exogenous fragments, low thioglycoside phenotype and stable inheritance. |
| priorityDate | 2019-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.