http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110484550-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-884 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 |
| filingDate | 2019-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110484550-B |
| titleOfInvention | Carotenoid metabolism gene function identification method |
| abstract | The invention discloses a carotenoid metabolic gene function identification method, which comprises the following steps: s1: cloning of the selectable marker elements: cloning of the plasmid from pGBK-T7 by Amp r Promoter-driven kanamycin resistance gene Kan r With this element (pAmp) r ::Kan r ) As a subsequent screening marker; s2: cloning of the promoter: the promoter sequence of a RuBisCO large subunit gene (rbcL) in cyanobacteria Synechocystis sp.PCC6803 is cloned, and the promoter is used for driving an enzyme gene to be detected; s3: cloning of the enzyme genes: the carotenoid metabolism-related enzyme gene X to be identified from different organisms is cloned downstream of the rbcL promoter (rbcL:: X), driven by this promoter; s4 construction of the expression cassette: an entire gene expression cassette comprises a pAmp arranged in sequence r ::Kan r A selection marker element and an rbcL:: X element for expressing an enzyme protein. Compared with the traditional identification method, the method has the advantages of simplicity, convenience and directness, and can definitely and pertinently identify the gene function. |
| priorityDate | 2019-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.