http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110484505-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-734 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-385 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-405 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-155 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0221 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P25-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0619 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0793 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P25-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-30 |
| filingDate | 2019-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110484505-B |
| titleOfInvention | Motor neuron and preparation method and application thereof |
| abstract | The invention relates to the field of stem cell biology, in particular to a motor neuron and a preparation method and application thereof. The motor neuron expresses OLIG2+, MAP2+ and CHAT +, and also expresses one or any combination of PAX6+, HB9+, ISL1+, Synaptophysin + and Tubulin +. The invention also discloses a method for preparing the motor neuron, which comprises the following steps: differentiation of pluripotent stem cells into neuroepithelial cells; differentiation of neuroepithelial cells into motor nerve precursor cells; differentiation of motor nerve precursor cells to post-mitotic motor neurons; and (3) differentiating the post-mitotic motor neurons into mature motor neurons to obtain the mature motor neurons. The preparation method provided by the invention does not use a system containing serum or a serum substitute; the differentiation efficiency is high, and the differentiation effect is stable; the purification method is simple and convenient, and the obtained cells have high purity. The frozen stock solution of motor nerve precursor cells is used, and has clear components and low cost; after recovery, the cell viability is high (> 80%), and the ability of efficiently differentiating to mature motor neurons is still maintained. |
| priorityDate | 2019-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 223.