http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110484495-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0655 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
| filingDate | 2019-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110484495-B |
| titleOfInvention | Method for separating and culturing cells from synovial articular cartilage of old people |
| abstract | The invention relates to a method for separating and culturing cells from synovial articular cartilage of old people, belonging to the technical field of human tissue engineering. It can obtain a great amount of cells with metabolic activity from the old joint cartilage, and is applied to the molecular pathology and biological treatment basic research of clinical autologous chondrocyte transplantation for repairing cartilage tissue defect and cartilage degenerative lesion. The chondrocyte separation method combines mechanical cutting and enzymolysis of cartilage tissues, and leads cartilage fragments to be fully contacted with the specific protease of degradable cartilage extracellular matrix protein in a tissue decomposition bottle (Spinner flash) and a cell culture box sterilized at high temperature and high pressure, thereby achieving the purpose of completely dissolving the matrix and releasing the chondrocytes to the maximum extent. Compared with the existing method for separating the chondrocytes of the human load-bearing synovial joint, the method has the advantages that the yield of the chondrocytes is higher, the proportion of the viable chondrocytes is higher, and the pollution probability is lower. |
| priorityDate | 2019-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.