http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110484491-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0692 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074 |
| filingDate | 2019-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110484491-B |
| titleOfInvention | Method for obtaining amniotic membrane and amniotic fluid derived endothelial progenitor cells and purification culture method thereof |
| abstract | The invention discloses a method for obtaining endothelial progenitor cells from human and mammal amniotic membrane and amniotic fluid, which comprises the following steps: pretreating the amnion stripped from the placenta; mechanically removing the pretreated sponge layer and epithelial layer of the amniotic membrane to obtain an amniotic membrane tissue; cutting the amniotic tissue into a preset size, mixing the cut amniotic tissue with a collagenase IV solution, digesting, and discarding the supernatant obtained by digestion; and mixing the amniotic membrane tissue after the first digestion step with a fresh collagenase IV solution again, digesting, centrifuging, and discarding the supernatant to obtain the endothelial progenitor cells. The amniotic membrane tissue block does not need to be ground and filtered, epithelial cells and mesenchymal stem cells in the amniotic membrane are fully removed by mechanical scraping and a gradient stepwise digestion method, and the technical problem that high-purity endothelial progenitor cells cannot be obtained from the amniotic membrane tissue in the prior art is solved. |
| priorityDate | 2019-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 159.