http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110467662-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fd07017c06b2a16178efe65c3c872775 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-461 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 |
| filingDate | 2019-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_457d83549991fb78dc914397a15097ca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97601491f2e962bbc9e49dbe82a62788 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56f392167df0394ef7b35c14f5a6b21c |
| publicationDate | 2019-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110467662-A |
| titleOfInvention | A kind of preparation method of tuna nucleoprotamine |
| abstract | Preparation method of the present invention about a kind of tuna nucleoprotamine, comprising: 1) tuna spermary is homogenized, and is impregnated with ethyl acetate, filtering drying and crushes after stirring；2) mixed solution of NaCl and sodium citrate are added in tuna milt dried powder, is stirred at low speed after homogeneous homogenate, is centrifuged at a high speed after standing and takes precipitating；3) sulfuric acid solution is added in the sediment of step 2) to extract, supernatant liquid filtering；With cold ethanol precipitation, crude product is freeze-dried to obtain after precipitating acetone washing；4) crude product wiring solution-forming injection CM Sephaorse CL-6B, which is purified by flash, is freeze-dried up to sterling.The method of the present invention prepares tuna nucleoprotamine yield up to 15% or more, sterling protein concentration is high, total amino acid content is up to 77% or so, potency is probably the 2/3 of mark product reference substance in nucleoprotamine and when heparin, RSD is measured less than 5%, the regulation for meeting pharmacopoeia of each country can be used as safe heparin antidote. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116179638-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116179638-B |
| priorityDate | 2019-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 131.