http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110452923-A

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filingDate 2019-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9203977d91f6c086e09dc1029c7bf4f3
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publicationDate 2019-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110452923-A
titleOfInvention NCD-2 bacterial strain is from constitutive promoter and its preparation method and application
abstract The present invention relates to Bacillus subtillis NCD-2 bacterial strains from constitutive promoter and its application.Bacillus subtillis NCD-2 bacterial strain of the present invention is from the sequence of constitutive promoter as shown in sequence table SEQ ID No:1.The present invention screens the promoter sequence of constitutive expression in NCD-2 bacterial strain using shuttle vector pAD123;According to the promoter of constitutive expression in the artificial synthesized NCD-2 bacterial strain of gained promoter sequence;Can be with construction of expression vector using this promoter, and the expression vector that builds can be imported in host cell, recombinant bacterial strain is constructed with this;This bacterial strain can form the interconnection with three-dimensional structure and relatively fixed biofilm structure, can be used for observing Bacillus subtillis NCD-2 bacterial strain using the NCD-2 bacterial strain that the GFP is marked and colonize situation in plant root.
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