Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_66729f7638e131330b02514cc2712f18 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01G7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01G22-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01G31-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01G22-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01G7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01G31-00 |
filingDate |
2019-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9203977d91f6c086e09dc1029c7bf4f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_217940be5c3b81e158df5803a76960c2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_20bf2e26a166a930a21a3b065e833094 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f933a78b2e4fed79cc0ae42160f34afc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01cb61c97c51c78cee2d19bd95743042 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4fbd043db446a95f456a1ae496c41845 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b411d635bc7f1cba217d70d5ff1819f2 |
publicationDate |
2019-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-110452923-A |
titleOfInvention |
NCD-2 bacterial strain is from constitutive promoter and its preparation method and application |
abstract |
The present invention relates to Bacillus subtillis NCD-2 bacterial strains from constitutive promoter and its application.Bacillus subtillis NCD-2 bacterial strain of the present invention is from the sequence of constitutive promoter as shown in sequence table SEQ ID No:1.The present invention screens the promoter sequence of constitutive expression in NCD-2 bacterial strain using shuttle vector pAD123;According to the promoter of constitutive expression in the artificial synthesized NCD-2 bacterial strain of gained promoter sequence;Can be with construction of expression vector using this promoter, and the expression vector that builds can be imported in host cell, recombinant bacterial strain is constructed with this;This bacterial strain can form the interconnection with three-dimensional structure and relatively fixed biofilm structure, can be used for observing Bacillus subtillis NCD-2 bacterial strain using the NCD-2 bacterial strain that the GFP is marked and colonize situation in plant root. |
priorityDate |
2019-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |