http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110452874-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0619 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0793 |
| filingDate | 2019-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110452874-B |
| titleOfInvention | Method for obtaining neuron |
| abstract | The invention provides a method for obtaining neurons, which belongs to the technical field of biological medicines and comprises the following steps: 1) mixing the nerve nucleus and papain, carrying out enzymolysis and blow beating on the obtained mixture to obtain filtrate; the enzyme activity unit of the papain in the mixture is 18-22U/ml; 2) mixing the filtrate obtained in the step 1) with erythrocyte lysate, and then cracking to obtain a lysate; 3) centrifuging the lysate obtained in the step 2) to obtain a precipitate as neurons. The nerve nucleus is subjected to enzymolysis through papain to release neurons, the obtained filtrate contains red blood cells besides the neurons, and the red blood cells are cracked by red blood cell cracking liquid and then centrifuged to remove cell fragments, so that the neurons are obtained. The average cell body diameter of the neuron is 10 μm, the activity is high, the clustering rate is low, and the method is suitable for 10 x single cell sequencing. |
| priorityDate | 2019-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.