abstract |
The present invention provides anti-VEGF single domain antibodies and applications thereof. Specifically, the present invention provides an anti-VEGF single domain antibody and its VHH chain. The present invention also provides a coding sequence encoding the single domain antibody or its VHH chain, a corresponding expression vector, a host cell capable of expressing the single domain antibody, and a method for producing the single domain antibody of the present invention. The single-domain antibody of the present invention can specifically recognize human VEGFA, does not cross-react with VEGFB, VEGFC and VEGFD, and has good specificity; the single-domain antibody of the present invention can simultaneously recognize VEGFA of human, mouse, rabbit and monkey, and can effectively block Interaction between VEGFA and VEGFR2, VEGFA and VEGFR1; and has excellent inhibitory effect on angiogenesis. The single-domain antibody of the present invention is easy to generate. |