http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110447542-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_98d52dbd560ffbacc2e86e336d0e0b58 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
filingDate | 2019-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_efce5d8a228a1842eac2f4bc12aa08a8 |
publicationDate | 2019-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110447542-A |
titleOfInvention | A kind of tissue culture and rapid propagation method of Chinese medicine Fourstamen Stephania Root |
abstract | The present invention relates to the methods of Plant Tissue Breeding in agricultural biotechnologies, specifically, being related to a kind of tissue culture and rapid propagation method of Chinese medicine Fourstamen Stephania Root, it is intended to solve the disadvantages such as prior art cutting propagation is long there are the period, breeding coefficient is low.The tissue culture and rapid propagation method includes the following steps successively carried out: obtaining explant, explant Fiber differentiation, Multiplying culture, culture of rootage and the transplanting of Fourstamen Stephania Root plant;It is 5.6~5.9 that the explant induced medium of the described explant Fiber differentiation operation, which includes: WPM culture medium, 1.5~2mg/L ZT, 0.2~0.4mg/L GA, 10~20mg/L CH, 25~30g/L sucrose, 3.0~4.0g/L agar, 100~500mg/L PVP, pH,.The present invention selects Fourstamen Stephania Root stem-segment with node for explant, through Fiber differentiation, Multiplying culture, culture of rootage and transplanting and other steps, inductivity has reached 90% or more, and survival rate has reached 90% or more, the tissue-culturing rapid propagation of Fourstamen Stephania Root is realized, to realize the purpose of the present invention. |
priorityDate | 2019-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.