http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110438124-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-708 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2019-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110438124-B |
| titleOfInvention | High-throughput detection probe, melting curve detection method and application thereof |
| abstract | The invention discloses a high-throughput detection probe and a melting curve detection method and application thereof, the probe of the invention forms a molecular beacon probe with a similar hairpin structure in an amplification stage, the probe quenches by using the fluorescence of a plurality of G base pair probes, the probe is melted with target nucleic acid in a subsequent melting program segment, a Tm value is adjusted according to the base composition of a 5' tail sequence of the probe or the extension length of a downstream region of an amplicon, the probe can be marked by multicolor fluorescent groups, and the aim of higher-throughput detection is achieved by using multiple channels, wherein a single channel at least detects 8 target nucleic acids; when the detection probe is subjected to first round amplification, the target sequence specific binding sequence I is specifically bound with the target nucleic acid, the target sequence specific binding sequence II is subjected to enzyme digestion to form a second round extended primer, and through twice specific binding, the detection specificity is enhanced, a narrower melting peak is formed, the channel utilization efficiency is improved, and the detection sensitivity is also improved. |
| priorityDate | 2019-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 19.