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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
filingDate 2019-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-110423807-B
titleOfInvention Primer combination and kit for detecting deletion type alpha-thalassemia
abstract The invention relates to an in-vitro molecular diagnosis technology, in particular to a primer combination and a kit for detecting deletion type alpha-thalassemia. The primer combination can stably detect-alpha 3.7 And- (alpha) 20.5 The two deletion types overcome the problems of unstable amplification system due to high GC content of the alpha-globin gene region; meanwhile, the annealing temperatures of the primers are in the same temperature range, so that the phenomena of nonspecific amplification and unequal efficiency caused by different annealing temperatures are avoided, in addition, the compatibility of the primers is good, no dimer is generated, the kit overcomes the influence among the primers, the multiple single-tube thalassemia of 7 genotypes can be detected, the requirements of clinical rapid and convenient detection of thalassemia are met, conditions are created for more comprehensive thalassemia screening, and scientific basis is provided for prenatal examination and thalassemia diagnosis of pregnant fetuses.
priorityDate 2019-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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