| abstract |
The invention provides a method for preparing Jo-1 protein and its application, and relates to the technical field of protein engineering. The preparation method of the Jo-1 protein comprises using pET-28a to express a Jo-1 fusion protein with an MBP tag, and then cutting off the tag during protein purification to obtain the Jo-1 protein. The preparation method has high production efficiency, and the prepared Jo-1 protein is close to the natural protein, so it can be used as the Jo-1 antigen to prepare Jo-1 antibodies, or to prepare products for diagnosing Jo-1-related diseases. |