| abstract |
Provided herein are cell surface conjugates comprising cell surface molecules and at least one agent, such as at least one affinity tag, as well as engineered cells expressing such cell surface conjugates. In some embodiments, the cell surface molecule does not contain an intracellular signaling domain or is incapable of mediating intracellular signaling. In some embodiments, cells, such as T cells, engineered to contain the cell surface conjugate further contain a genetically engineered recombinant receptor, such as a chimeric antigen receptor (CAR), that specifically binds an antigen. Also provided are methods of detecting, identifying, selecting or targeting cells expressing the cell surface conjugates, for example, in conjunction with methods of making engineered cells or in conjunction with administering such cells to a subject, including adoptive methods of cell therapy. |