http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110408645-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-30 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 |
filingDate | 2019-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-110408645-B |
titleOfInvention | Multiple traceless integration system and method for target gene in yarrowia lipolytica |
abstract | The invention relates to a multiple traceless integration system and a multiple traceless integration method for a target gene in yarrowia lipolytica, and belongs to the technical field of genetic engineering. The system can introduce lox sites by transforming the yarrowia lipolytica through the plasmid 1, and then can realize the traceless integration of a target gene into the yarrowia lipolytica genome at any times through the sequential transformation of the plasmid 2 and the plasmid 3. The integration of a target gene for any number of times can be realized only by one yeast screening marker, meanwhile, the introduction of non-target genes is greatly reduced, and the genome after each integration is known and controllable, so that the application of a yarrowia lipolytica expression system in the fields of food, medicine, agricultural product processing and the like is facilitated. |
priorityDate | 2019-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.