http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110408607-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2474 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01035 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-25 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-26 |
| filingDate | 2019-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110408607-B |
| titleOfInvention | Fermentation optimization process for producing hyaluronidase by lactobacillus plantarum |
| abstract | The invention relates to the technical field of microbial fermentation, in particular to a fermentation optimization process for producing hyaluronidase by lactobacillus plantarum, which comprises the following steps: preparing a seed culture medium; inoculating lactobacillus plantarum into a seed culture medium, and culturing to obtain a liquid fermentation strain; preparing a liquid fermentation culture medium; inoculating the liquid fermentation strain into a liquid fermentation culture medium, and finishing fermentation after culture; centrifuging the fermentation liquor, and collecting supernatant to obtain crude enzyme solution. The invention creatively carries out the acclimation culture of the oxygen resistance of the bacteria by adjusting the fermentation culture rotating speed and the ventilation quantity, so that the lactobacillus plantarum can normally ferment and produce the hyaluronidase under the aerobic condition; through condition optimization, the yield of hyaluronidase in the lactobacillus plantarum metabolites is greatly improved: the hyaluronidase contained in the fermentation liquor prepared by the production process is 9604IU/mL, which is improved by 40 percent compared with that before the process optimization, thereby facilitating the subsequent separation and purification; the invention has simple and convenient production process and high stability, and is convenient for large-scale production and popularization. |
| priorityDate | 2019-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.