http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110387346-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-625 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01075 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01036 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1029 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-18 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-62 |
| filingDate | 2019-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-110387346-B |
| titleOfInvention | Gene engineering bacterium lacking 21 coding exopolysaccharide synthetic genes and application thereof |
| abstract | The invention discloses a gene engineering bacterium lacking 21 coding extracellular polysaccharide synthetic genes and application thereof, belonging to the field of gene engineering and fermentation engineering. The invention uses CRISPR/Cas9 knock-out system to knock out 21 genes which are responsible for synthesis and transportation of extracellular polysaccharide on Escherichia coli W3110 genome to obtain mutant bacteria WJW09, uses WJW09 strain as host bacteria, uses pBHR68 to heterologously express PHB synthetic gene cluster phaCAB, the PHB synthesized by WJW09/pBHR68 can reach 57.2% of cell dry weight, and the PHB synthesized by W3110/pBHR68 only accounts for 1.5% of the cell dry weight. The recombinant strain WJW09/pBHR68 has PHB volume yield 38 times higher than that of control strain W3110/pBHR68 (1.5%). |
| priorityDate | 2019-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 80.